Aggregation induced emission-based materials for fluorescence imaging of latent fingerprints using wet and powder dusting methods: Effect of physical factors and tape lifting.

• The solvatochromic HBT1 dye which harbours aggregation induced emission in > 80 % H 2 O–CH 3 CN solution has been utilized for fluorescence imaging of Latent fingerprints (LFPs) using wet and powder dusting methods. • We demonstrate the effectiveness of HBT1 as a fingerprint solution and powder on porous, semi porous and non-porous surfaces for visualization of levels 1–3 including details of sweat pores. • We have carried out the effect of physical factors such as temperature (25–300 °C) and humidity (40–80%) on fingerprint development. • The lifting of developed fingerprints using adhesive tape and dactyloscopy (analysis of fingerprints) have been demonstrated. • The agarose gel electrophoresis revealed that HBT1 does not degrade the dsDNA at a concentration as high as 1 mM (dsDNA: HBT1 ratio 1:15000). The solvatochromic HBT1 dye which harbours aggregation induced emission in > 80 % H 2 O–CH 3 CN solution and solid-state emission properties has been utilized for fluorescence imaging of Latent fingerprints (LFPs) using wet and powder dusting methods. We demonstrated (i) effectiveness of HBT1 as a fingerprint powder on porous, semi porous and non-porous surfaces; (ii) visualization of levels 1–3 including details of sweat pores; (iii) effect of temperature (25–300 °C) and humidity (40–80 %) on fingerprint development; (iv) lifting of developed fingerprints using adhesive tape; (v) development of old or aged (90 days) and stained LFPs and (vi) dactyloscopy (analysis of fingerprints of different subjects). The gel electrophoresis study showed no degradation of dsDNA in the presence of HBT1 at 0.01–1 mM concentration. [ABSTRACT FROM AUTHOR]