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Accurate neuron segmentation method for one-photon calcium imaging videos combining convolutional neural networks and clustering.
- Source :
-
Communications Biology . 8/9/2024, Vol. 7 Issue 1, p1-16. 16p. - Publication Year :
- 2024
-
Abstract
- One-photon fluorescent calcium imaging helps understand brain functions by recording large-scale neural activities in freely moving animals. Automatic, fast, and accurate active neuron segmentation algorithms are essential to extract and interpret information from these videos. One-photon imaging videos' low resolution, high noise, and high background fluctuation pose significant challenges. Here, we develop a software pipeline to address the challenges of processing one-photon calcium imaging videos. We extend our previous two-photon active neuron segmentation algorithm, Shallow U-Net Neuron Segmentation (SUNS), to better suppress background fluctuations in one-photon videos. We also develop additional neuron extraction (ANE) to locate small or dim neurons missed by SUNS. To train our segmentation method, we create ground truth neurons by developing a manual labeling pipeline assisted with semi-automatic refinement. Our method is more accurate and faster than state-of-the-art techniques when processing simulated videos and multiple experimental datasets acquired over various brain regions with different imaging conditions. SUNS2-ANE segments active neurons from one-photon calcium imaging videos accurately and fast. It improves background suppression and extracts additional weak neurons. A separate semi-automatic labeling pipeline generates ground truth for training. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 23993642
- Volume :
- 7
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Communications Biology
- Publication Type :
- Academic Journal
- Accession number :
- 178954735
- Full Text :
- https://doi.org/10.1038/s42003-024-06668-7