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Asymmetric Sulfoxidations Catalyzed by Bacterial Flavin-Containing Monooxygenases.

Authors :
de Gonzalo, Gonzalo
Coto-Cid, Juan M.
Lončar, Nikola
Fraaije, Marco W.
Source :
Molecules. Aug2024, Vol. 29 Issue 15, p3474. 10p.
Publication Year :
2024

Abstract

Flavin-containing monooxygenase from Methylophaga sp. (mFMO) was previously discovered to be a valuable biocatalyst used to convert small amines, such as trimethylamine, and various indoles. As FMOs are also known to act on sulfides, we explored mFMO and some mutants thereof for their ability to convert prochiral aromatic sulfides. We included a newly identified thermostable FMO obtained from the bacterium Nitrincola lacisaponensis (NiFMO). The FMOs were found to be active with most tested sulfides, forming chiral sulfoxides with moderate-to-high enantioselectivity. Each enzyme variant exhibited a different enantioselective behavior. This shows that small changes in the substrate binding pocket of mFMO influence selectivity, representing a tunable biocatalyst for enantioselective sulfoxidations. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14203049
Volume :
29
Issue :
15
Database :
Academic Search Index
Journal :
Molecules
Publication Type :
Academic Journal
Accession number :
178948678
Full Text :
https://doi.org/10.3390/molecules29153474