Compound ANBP promotes diabetic wound healing by regulating TCA circulation-related enzymes.

AIM: To investigate the effects of the compound ANBP on wound healing in diabetic rats and explore its mechanism of action. METHODS: Ninety male SD rats were randomly divided into blank, model, compound ANBP, Beifuxin, and nicotinamide mononucleotide (NMN) groups, with 16 rats in each group. Wound healing in each group was observed and samples were taken on days 3, 7 and 14 to analyze the wound healing rate. Local histopathological changes were observed using HE and Masson staining. The expressions of pyruvate dehydrogenase E1 subunit alpha 1 (PDHAl), citrate synthase (CS), isocitrate dehydrogenase (IDH1) and oxoglutarate dehydrogenase (OGDH) were detected through immunofluorescence and Western blot. The number and morphology of mitochondria in the wound tissue were observed using transmission electron microscopy. RESULTS: Histomorphological changes revealed significant improvement in diabetic wound healing in the blank and compound ANBP groups compared to that of the model group. The wound healing rates of the blank, compound ANBP, Beifuxin, and NMN groups were significantly increased on days 3, 7, and 14 (P<0. 01). Compared to the model group, granulation tissue generation was higher in the other groups, covering the wound defect and producing abundant collagen fibers. At 3, 7, and 14 days after intervention, the blank, compound ANBP, Beifuxin, and NMN groups showed significantly enhanced fluorescence intensities of TCA cycling-related enzymes PDHA1, CS, IDH1, and OGDH indicating increased expression of these enzymes. The levels of the TCA cycling-related enzymes were significantly increased (P<0. 01) in the compound ANBP, Beifuxin and NMN groups but were significantly decreased (P<0. 01) in the model group. An increase in the number and density of mitochondria and a decrease in the cavitation rate of mitochondria with improved morphology (P<0. 05) was observed in the group treated with compound ANBP. CONCLUSION: Compound ANBP may increase the number of mitochondria, improve mitochondrial morphology and function, upregulate the expression levels of PDHA1, CS, IDH1, and OGDH proteins, and accelerate the regeneration of wound granulation tissue, thus promoting the healing of diabetic wounds in rats. [ABSTRACT FROM AUTHOR]