Eco-scale based greenness assessment of a validated multi-residue method for the quantification of 103 pesticides in cookies sample.

A robust QuEChERS coupled LC-ESI-MS/MS method for the detection and quantification of 103 fortified pesticides in cookies was developed. Pre-soaking, before extraction assisted in releasing the adsorbed pesticides from the matrix. Original QuEChERS with little modification, and a 25 mg C-18+150 mg anhydrous MgSO 4 combination yielded acceptable recovery for 98 pesticides. The method was validated for SANTE guidelines (SANTE/11312/2021). 95, 80 and 60 pesticides could be satisfactorily recovered at 0.1, 0.05, and 0.01 µg g−1 fortification with HorRat values 0.3 – 1. The total uncertainty of the method ranged between 5.55 % and 28.73 % with a mild to medium matrix effect for the majority. Residue analysis in real samples was carried out and the detected residues were less than the prescribed MRLs in raw wheat grain. According to the analytical Eco-Scale protocol, the method had penalty points of 23 and thus is much greener and more environmentally friendly with the overall eco-score of 77. [Display omitted] • The multi residue LC-MS/MS method for the detection and quantification of 103 pesticides in cookies. • Original QuEChERS extraction with little modification acceptable recovery for 98 pesticides with <20 % RSD. • Method validation and real sample analysis. • Eco-Scale based greenness assessment using penalty points. [ABSTRACT FROM AUTHOR]