Tuber quality enhancement via grafting potato onto a wooden goji rootstock through vitalizing multi-pathways.

Grafting is applied in Solanaceae to improve growth and quality traits. However, grafting potato onto a wooden goji rootstock is rare. Our study introduces a novel distant grafting technique to investigate potato scion responses, specifically regarding photosynthetic and tuber nutritional quality. The physiological and transcriptomic findings reveal an increase in photosynthesis ratio and carbon fixation in potato leaves after 45 days of grafting due to the upregulation of pivotal genes (PsbA , PPC1 , rbcl, and GAPDH). After 95 days of long-term growth, the leaf redox balance was maintained with intensified chlorophyll synthesis, facilitated by the enrichment of crucial genes (GUN4 , CHLH , CHLP , CAO) and several light-harvesting proteins (Lhca and Lhcb) in potato leaves. The tubers of grafted plants showed a 6.5% increase in crude protein, 51% in anthocyanin, and lower carbohydrate content. Goji altered the expression of tubers genes involved in assimilatory sulfate reduction, which subsequently affects cysteine-methionine biosynthesis. Furthermore, the tuber transcriptome shows ABA signaling and transcription factors regulate the expression of key biosynthetic genes involved in inducing the secondary metabolites, such as scopoletin and anthocyanin accumulation, which are primary polyphenols in goji. Our innovative grafting approach offers valuable insights into the interactions between woody and herbaceous plants for developing future strategies to modulate growth efficiency and tuber quality in the face of climate challenges and to meet the demand for nutritious food. [Display omitted] • Goji rootstock enhances chlorophyll, PSII efficiency and delay senescence. • Harvested tubers had higher anthocyanin, protein, and a lower carbohydrate content. • Sulfur assimilation is incorporated into amino acid synthesis and enzymes activity. • ABA signaling in tubers activates TFs that regulate quality and dormancy. • Upregulation of 3RT and BAHD- 5AT enhanced the anthocyanins biosynthesis. [ABSTRACT FROM AUTHOR]