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UV mutagenesis for lipase overproduction from Bacillus cereus ATA179, nutritional optimization, characterization and its usability in the detergent industry.

Authors :
Irianto, Vichi Sicha
Demirkan, Elif
Cetinkaya, Aynur Aybey
Source :
Preparative Biochemistry & Biotechnology. Aug2024, Vol. 54 Issue 7, p918-931. 14p.
Publication Year :
2024

Abstract

In this study, the wild-type Bacillus cereus ATA179 was mutagenized by random UV mutagenesis to increase lipase production. The mutant with maximum lipolytic activity was named Bacillus cereus EV4. The mutant strain (10.6 U/mL at 24 h) produced 60% more enzyme than the wild strain (6.6 U/mL at 48 h). Nutritional factors on lipase production were investigated. Sucrose was the best carbon source, (NH4)2HPO4 was the best nitrogen source and CuSO4 was the best metal ion source. Mutant EV4 showed a 32% increase in lipase production in the modified medium. The optimum temperature and pH were found to be 60 °C and 7.0, respectively. CuSO4, CaCl2, LiSO4, KCl, BaCl2, and Tween 20 had an activating effect on the enzyme. Vmax and Km values were found to be 17.36 U/mL and 0.036 mM, respectively. The molecular weight was determined as 28.2 kDa. The activity of lipase was found to be stable up to 60 days at 20 °C, 75 days at 4 °C, and 90 days at −20 °C. The potential of lipase in the detergent industry was investigated. The enzyme was not affected by detergent additives but was effective in removing stains in fabrics contaminated with oily substances. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10826068
Volume :
54
Issue :
7
Database :
Academic Search Index
Journal :
Preparative Biochemistry & Biotechnology
Publication Type :
Academic Journal
Accession number :
178808327
Full Text :
https://doi.org/10.1080/10826068.2023.2299441