A ratiometric fluorescent probe based on UiO-66-TCPP for selective and visual detection of quercetin in food.

Quercetin (QCT) is a flavonoid with significant health benefits, necessitating sensitive detection methods for food safety and quality control. This study presents a novel UiO-66-TCPP ratiometric fluorescent probe for the quantitative and visual detection of QCT. Under optimal conditions, the fluorescence intensity of UiO-66-TCPP decreased linearly with increasing QCT concentration, with a detection limit of 26 nM. The probe demonstrated high specificity, showing no significant interference from various substances and QCT analogues. Practical applicability was confirmed by testing artificially contaminated juice samples, achieving recovery rates between 98.0% and 104.8%. Furthermore, a paper-based sensor was developed by incorporating UiO-66-TCPP onto Whatman#1 chromatography paper. This sensor exhibited stable fluorescence and a reliable, sensitive visual response to QCT concentrations, detectable via a smartphone-based color recognizer application. The UiO-66-TCPP ratiometric fluorescent probe provides a sensitive, specific, and practical method for detecting QCT in food matrices, offering significant potential for both laboratory and on-site applications. • A ratiometric fluorescent probe, namely UiO-66-TCPP, was synthesized for the detection of quercetin. • A paper-based sensor utilizing UiO-66-TCPP was fabricated for convenient on-site detection. • The ratiometric fluorescent probe exhibited remarkable sensitivity, with a detection limit as low as 26 nM. [ABSTRACT FROM AUTHOR]