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Glycolysis Changes in Alloreactive Memory B Cells in Highly Sensitized Kidney Transplant Recipients Undergonig Desensitization Therapy.
- Source :
-
Transplant International . 2024, p1-12. 12p. - Publication Year :
- 2024
-
Abstract
- Despite the growing use of desensitization strategies, hyperimmune patients remain at high risk of antibody-mediated rejection suggesting that, even when donor-specific antibodies (DSA) are effectively depleted, anti-donor specific B cells persist. We included 10 highly sensitized recipients that underwent desensitization with plasmapheresis and B cell depletion prior to kidney transplantation. We quantified changes in DSA (luminex), total B-cell subsets (flow cytometry), anti-donor HLA B cells (fluorospot), and single-cell metabolism in serially collected samples before desensitization, at the time of transplant, and at 6 and 12 months thereafter. Desensitization was associated with a decrease in DSA and total memory B cell and naive B cell percentage, while plasma cells and memory anti-donor HLA circulating B cells persisted up to 12 months after transplant. At 12-month post-transplantation, memory B cells increased their glycolytic capacity, while proliferative KI67+ plasma cells modified their metabolism by increasing fatty acid and amino acid oxidation capacity and decreasing their glucose dependence. Despite effective DSA depletion, anti-donor B cells persist in kidney transplant recipients. Due to the reliance of these cells on glycolysis, glycolysistargeting therapies might represent a valuable treatment strategy. [ABSTRACT FROM AUTHOR]
- Subjects :
- *IMMUNOLOGIC memory
*CELL metabolism
*B cells
*PLASMA cells
*GRAFT rejection
Subjects
Details
- Language :
- English
- ISSN :
- 09340874
- Database :
- Academic Search Index
- Journal :
- Transplant International
- Publication Type :
- Academic Journal
- Accession number :
- 178707420
- Full Text :
- https://doi.org/10.3389/ti.2024.13029