Maintenance of Lognormal‐Like Skewed Dendritic Spine Size Distributions in Dentate Granule Cells of TNF, TNF‐R1, TNF‐R2, and TNF‐R1/2‐Deficient Mice.

Dendritic spines are sites of synaptic plasticity and their head size correlates with the strength of the corresponding synapse. We recently showed that the distribution of spine head sizes follows a lognormal‐like distribution even after blockage of activity or plasticity induction. As the cytokine tumor necrosis factor (TNF) influences synaptic transmission and constitutive TNF and receptor (TNF‐R)‐deficiencies cause changes in spine head size distributions, we tested whether these genetic alterations disrupt the lognormality of spine head sizes. Furthermore, we distinguished between spines containing the actin‐modulating protein synaptopodin (SP‐positive), which is present in large, strong and stable spines and those lacking it (SP‐negative). Our analysis revealed that neither TNF‐deficiency nor the absence of TNF‐R1, TNF‐R2 or TNF‐R 1 and 2 (TNF‐R1/R2) degrades the general lognormal‐like, skewed distribution of spine head sizes (all spines, SP‐positive spines, SP‐negative spines). However, TNF, TNF‐R1 and TNF‐R2‐deficiency affected the width of the lognormal distribution, and TNF‐R1/2‐deficiency shifted the distribution to the left. Our findings demonstrate the robustness of the lognormal‐like, skewed distribution, which is maintained even in the face of genetic manipulations that alter the distribution of spine head sizes. Our observations are in line with homeostatic adaptation mechanisms of neurons regulating the distribution of spines and their head sizes. [ABSTRACT FROM AUTHOR]