Back to Search
Start Over
Remimazolam protects the liver from ischemia-reperfusion injury by inhibiting the MAPK/ERK pathway.
- Source :
-
BMC Anesthesiology . 7/25/2024, Vol. 24 Issue 1, p1-8. 8p. - Publication Year :
- 2024
-
Abstract
- Background: Ischemia-reperfusion (I/R) injury is a major factor in liver damage following hepatic resection and liver transplantation, with anesthetics demonstrating the ability to shield organs from this type of injury. Methods: Hypoxia-reoxygenation (H/R) was used to create in vitro I/R hepatocyte cell injury models. The CCK-8 assay, flow cytometer, LDH assay, and ELSIA were utilized to assess hepatocyte injury. The in vivo I/R injury rat model was then built. HE and TUNEL staining were used to assess liver tissue damage. Western-blot was applied to assess the activation of the MAPK/ERK pathway. Results: Remimazolam (RMZL) remarkably improved cell viability and decreased apoptosis in H/R-induced hepatocyte injury. RMZL reduced the release of H/R-induced inflammatory mediators (TNF-α and IL-6) as well as LDH levels. We also discovered that RMZL inhibited p38 and ERK1/2 phosphorylation in vivo and in vitro. The stimulation of MAPK/ERK, on the other hand, abolished RMZL's anti-inflammation effects in H/R-induced hepatocyte injury. Furthermore, RMZL reduced liver tissue injury in I/R rats. Conclusion: RMZL prevented hepatic I/R damage by inhibiting MAPK/ERK signaling. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ISCHEMIA prevention
*LIVER injuries
*LIVER disease prevention
*BENZODIAZEPINES
*MITOGEN-activated protein kinases
*IN vitro studies
*BIOLOGICAL models
*FLOW cytometry
*REPERFUSION injury
*RESEARCH funding
*INFLAMMATORY mediators
*PHOSPHORYLATION
*ENZYME-linked immunosorbent assay
*APOPTOSIS
*TRANQUILIZING drugs
*CELLULAR signal transduction
*IN vivo studies
*LACTATE dehydrogenase
*RATS
*LIVER cells
*ANIMAL experimentation
*WESTERN immunoblotting
*LIVER
*TRANSFERASES
*STAINS & staining (Microscopy)
*CELL survival
*TUMOR necrosis factors
*INTERLEUKINS
*PHARMACODYNAMICS
*CHEMICAL inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 14712253
- Volume :
- 24
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BMC Anesthesiology
- Publication Type :
- Academic Journal
- Accession number :
- 178621277
- Full Text :
- https://doi.org/10.1186/s12871-024-02641-3