香菇多糖对ApoE-/-小鼠动脉粥样硬化斑块形成及AMPK 信号通路的影响.

Objective: To investigate effects of lentinan（ LNT） on atherosclerosis（ AS） plaque formation and adenosine 5'-mo-nophosphate-activated protein kinase （AMPK） signaling pathway in ApoE-/-mice. Methods: ApoE-/- mice were divided into model group， Lipitor group （5 mg/kg）， LNT low （5 mg/kg）， medium （10 mg/kg）， high （20 mg/kg） dose groups， C57BL/6 mice of similar genetic background were as control group. After 12 weeks of intragastric administration， automatic biochemical analyzer was used to detect serum triglycerides（ TG）， total cholesterol（ TC）， low-density lipoprotein（ LDL-C） and high-density lipoprotein（ HDL-C） levels； serum levels of IL-6 and IL-1β were detected by ELISA； oil red O staining was used to observe overall aortic plaque formation； HE staining was used to observe formation of plaque in aortic roots； AMPK pathway proteins expressions in aortawas were detected by Western blot. Results: Compared with control group， serum TC， TG， LDL-C， IL-6， IL-1β levels， aortic plaque area， aortic root plaque area， aortic tissue NLRP3， IL-6， IL-1β expressions were significantly increased in model group， p-AMPK/AMPK expression in aortic tissue and serum HDL-C level were reduced significantly （P<0.05）； compared with model group， TC， TG， LDL-C， IL-6， IL-1β， aortic plaque area， aortic root plaque area， aortic tissues NLRP3， IL-6 and IL-1β expressions were reduced significantly in Lipitor group and medium， high dose LNT groups， p-AMPK/AMPK expression in aortic tissue and serum HDL-C level were increased significantly， difference between each dose of LNT was statistically significant （P<0.05）； there was no significant difference between Lipitor group and LNT high-dose group（ P>0.05）. Conclusion: LNT may inhibit inflammatory response and formation of AS plaques in AS mouse by activating AMPK pathway. [ABSTRACT FROM AUTHOR]