MORPHOLOGICAL, PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF PSEUDOXANTHOMONAS SPECIES AND ITS OPTIMAL GROWTH KINETICS.

Pseudoxanthomonas species are candidate microorganisms with potential for biocontrol, therefore this study aimed to isolate and characterize a strain of Pseudoxanthomonas sp. by morphological, physiological and biochemical analysis. In addition, Pseudoxanthomonas sp. growth conditions were also optimized. The effects of different culture mediums, carbon sources, nitrogen sources and pH on the growth of Pseudoxanthomonas sp. were studied by a combination of univariate and orthogonal experiments to determine its optimal growth kinetics. The results showed that the isolated strains were gram-negative, pale yellow, round, raised, with clean edges, translucent and shiny. One of the best - growing representative strains was selected for further physiological and biochemical characterization and was identified as Pseudoxanthomonas sp. The optimal growth conditions for the representative strain were reported. It was found that using LB medium as the base medium, adding 1.5% glucose as the carbon source, 1.5% yeast extract as the nitrogen source, adjusting the medium pH to 7.5, and incubating at a temperature of 37? yielded the best growth kinetics. These findings hold significance for subsequent research endeavors. Specifically, they are pivotal for advancing the biotechnological development of Pseudoxanthomonas sp. [ABSTRACT FROM AUTHOR]