A Novel Analytical Method for Simultaneous Estimation of Andrographolide and Catechin in Polyherbal Formulation by High-Performance Liquid Chromatography.

Himalaya PartySmart capsule is a polyherbal formulation having liver-protective properties. The polyherbal capsule consists of extracts of six different herbs namely, Phoenix dactylifera, Cichorium intybus, Andrographis paniculata, Vitis vinifera, Phyllanthus amarus and Emblica officinalis. A reversed-phase high-performance liquid chromatography method with ultraviolet detection was developed to standardize commercial polyherbal capsule using andrographolide and catechin as markers. Two selected markers were separated on the C18 silica gel column by isocratic elution mode using methanol, phosphate buffer (potential of hydrogen 2.5) (70:30, v/v) as mobile phase at 27° and the detection wavelength was 259 nm. The method was validated as per the recommendations of the International Conference on Harmonization Q2 (R1) guidelines. Both the calibration curves exhibited a good linear coefficient of correlation (r2>0.995). The limit of detection and limit of quantitation values indicated that the developed method was sensitive even at low concentrations. The percent relative standard deviation of peak areas was less than 2 % for intra-day and inter-day precision. The recoveries of both markers ranged between 98 %-102 %. Two markers were quantified in polyherbal capsules containing andrographolide (0.72 %, w/w) and catechin (0.94 %, w/w). In conclusion, a specific, precise, accurate and robust reversed-phase high-performance liquid chromatography method was developed to determine andrographolide and catechin simultaneously which can be used to monitor the quality control studies. [ABSTRACT FROM AUTHOR]