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A systematic exploration of bacterial form I rubisco maximal carboxylation rates.
A systematic exploration of bacterial form I rubisco maximal carboxylation rates.
- Source :
-
EMBO Journal . Jul2024, Vol. 43 Issue 14, p3072-3083. 12p. - Publication Year :
- 2024
-
Abstract
- Autotrophy is the basis for complex life on Earth. Central to this process is rubisco—the enzyme that catalyzes almost all carbon fixation on the planet. Yet, with only a small fraction of rubisco diversity kinetically characterized so far, the underlying biological factors driving the evolution of fast rubiscos in nature remain unclear. We conducted a high-throughput kinetic characterization of over 100 bacterial form I rubiscos, the most ubiquitous group of rubisco sequences in nature, to uncover the determinants of rubisco's carboxylation velocity. We show that the presence of a carboxysome CO2 concentrating mechanism correlates with faster rubiscos with a median fivefold higher rate. In contrast to prior studies, we find that rubiscos originating from α-cyanobacteria exhibit the highest carboxylation rates among form I enzymes (≈10 s−1 median versus <7 s−1 in other groups). Our study systematically reveals biological and environmental properties associated with kinetic variation across rubiscos from nature. Synopsis: Form I rubisco, known for its slow kinetics in plants and algae, exhibits a great unexplored diversity in autotrophic bacteria. This article represents the first large-scale survey of bacterial form I rubisco kinetics and reveals unifying features of fast carboxylating rubiscos. Over 100 homologs were systematically screened, spanning the wide genetic diversity of form I rubisco enzymes across a variety of ecological niches and metabolic profiles. Phototrophy and carboxysome association are correlated with fast-carboxylating rubiscos. α-cyanobacteria emerges as the bacterial clade expressing the fastest form I rubiscos on Earth. Bacterial rubisco enzymes associated with carboxysomes have the fastest CO2-fixing rates. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02614189
- Volume :
- 43
- Issue :
- 14
- Database :
- Academic Search Index
- Journal :
- EMBO Journal
- Publication Type :
- Academic Journal
- Accession number :
- 178469273
- Full Text :
- https://doi.org/10.1038/s44318-024-00119-z