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A Simple Live Cell Imaging "Turn–On" Fluorescence Probe for the Selective and Sensitive Detection of Aqueous Hg2+ Ions.
- Source :
-
Journal of Fluorescence . Jul2024, Vol. 34 Issue 4, p1671-1682. 12p. - Publication Year :
- 2024
-
Abstract
- A simple, efficient, and reversible fluorescent sensor probe, PBA (2,6-dimethyl pyrone barbituric acid conjugate), comprised of a pro-aromatic donor conjugated with a barbituric acid, was developed for the detection of highly toxic mercuric ions. The probe showed high selectivity and "Turn-On" fluorescence response towards Hg2+ among various metal cations such as Na+, Mg2+, Ca2+, Mn2+, Fe2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+, Ba2+, Hg2+, and Pb2+, in both homogeneous and microheterogeneous micelle medium sodium dodecyl sulphate (SDS). The binding stoichiometry, limit of detection (LOD), and binding constant for the PBA-Hg complex were determined. The mechanism of binding was ascertained using the N,N'-dimethylbarbituric acid conjugate of 2,6-dimethylpyran (PDMBA), where no binding interaction by deprotonation is possible. In the presence of cysteamine hydrochloride and trifluoroacetic acid (TFA), the complexation of Hg2+ with PBA was demonstrated to be reversible, indicating its potential for the development of reusable sensors. Moreover, the practical applicability of PBA in monitoring Hg2+ in living cells was also evaluated. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10530509
- Volume :
- 34
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Fluorescence
- Publication Type :
- Academic Journal
- Accession number :
- 178444340
- Full Text :
- https://doi.org/10.1007/s10895-023-03390-1