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Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker.
- Source :
-
Talanta . Sep2024, Vol. 277, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy to amplify a specific DNA fragment of the chloroplast trnH-psbA. The procedure involves the selective capture of a 68-mer synthetic target DNA (or unmodified PCR products) through sandwich hybridization with RNA capture probe-modified streptavidin MBs and RNA signaling probes, labeled using antibodies specific to the heteroduplexes and secondary antibodies tagged with horseradish peroxidase. Amperometric measurements were performed on screen-printed electrodes using the H 2 O 2 /hydroquinone system. Achieving a LOD of 3 pM for the synthetic target, it was possible to detect 2.5 pg of peanut DNA and around 10 mg kg−1 of peanut in binary mixtures (defatted peanut flours prepared in spelt wheat). However, the detectability decreased between 10 and 1000 times in processed samples depending on the treatment. The Express PCR-bioplatform was applied to the detection of peanut traces in foodstuff. [Display omitted] • First disposable electrochemical nucleic acid sensor for peanut determination. • Sandwich methodology targeting a fragment of the peanut chloroplast DNA sequence. • Magnetic beads-assisted and amperometric transduction at screen-printed electrodes. • The bioplatform/Express PCR coupling allows detecting 10 mg kg−1 of peanut in food. • Successful interrogation of peanut traces in commercial food samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 277
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 178422935
- Full Text :
- https://doi.org/10.1016/j.talanta.2024.126350