Back to Search Start Over

Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens.

Authors :
Xue, Li
Zhu, Jianhong
Lei, Ke
Liu, Zeshi
Tang, Yiwei
Zhong, Bo
Gao, Ning
Xiong, Chaoliang
Lei, Jing
Tian, Ying
Zhou, Weixiao
Feng, Nan
Zhang, Xue
Chen, Dong
Li, Jing
Geng, Yan
Source :
Virology Journal. 7/11/2024, Vol. 21 Issue 1, p1-7. 7p.
Publication Year :
2024

Abstract

Objectives: The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed. Methods: Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method. Results: The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively. Conclusions: The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1743422X
Volume :
21
Issue :
1
Database :
Academic Search Index
Journal :
Virology Journal
Publication Type :
Academic Journal
Accession number :
178402388
Full Text :
https://doi.org/10.1186/s12985-024-02430-x