Cytotoxic activity of dichloromethane extract of soursop leaf (Annona muricata L.) against cancer.

Soursop leaves (Annona muricata) contain various secondary metabolite compounds with pharmacological benefits. This study foccused on the activity and chemical components cytotoxic active fraction of soursop leaf dichloromethane extract. From maceration of 535.8 g dried sample of A. muricata leaves we obtained extracts of n-hexane (18.4 g; 3.43 %). dichloromethane (11.4 g; 2.23 %). and methanol (48.7 g; 9.95 %). The dichloromethane extract was separated for its chemical components using gravity column chromatography resulting in 9 combined fractions. Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT) and cytotoxic test using MTT method 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide. The highest toxicity and cytotoxic test results were found in DMD 1 dichloromethane extract of A. muricata leaves with LC50 of 19.05 µg/mL and IC50 of 824.09 µg/mL. The results of DMD 1 gravity column re-chromatography produced 6-combined subfractions with the best separation pattern on DMD 1.4 and the results of GC-MS chromatogram analysis showed 29 peaks with the main component of fatty acid compounds namely Carbonic acid. but-2-yn-1-yl octadecyl ester (70.46 %). Potential anticancer compounds found were Phytol; 2-Hydroxy-1.1.10-trimethyl-6.9-epidioxydecalin and 2-Piperidinone. N-(4-bromo-n-butyl). [ABSTRACT FROM AUTHOR]