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QTL analysis of low-temperature tolerance in maize germination by SLAF-seq and BSA technique.
- Source :
-
Electronic Journal of Biotechnology . Jul2024, Vol. 70, p14-22. 9p. - Publication Year :
- 2024
-
Abstract
- Background: Cold damage of maize during germination is a global problem; it occurs frequently in northeast China, and leads to a large-scale reduction in yield. Low temperature tolerance of maize in germination is a complex quantitative trait controlled by multigenes, and no major QTLs or key genes have been identified. Results: An F2 isolation population with S319 and R144 as parents was constructed. The bulked segregant analysis (BSA) and specific-locus amplified fragment-sequencing (SLAF-seq) methods were applied to locate the chromosomal association regions related to low-temperature tolerance of maize during germination. Sequencing obtained 221.72 Gbp clean data, with an average sequencing depth of 25.96X. Four candidate regions associated with low-temperature tolerance trait of maize in germination were obtained, with a total length of 25.71 Mb and 1513 annotated genes, including 456 nonsynonymous mutant genes and 111 frameshift mutant genes. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GERMINATION
*DATA scrubbing
*LOW temperatures
*POUND sterling
Subjects
Details
- Language :
- English
- ISSN :
- 07173458
- Volume :
- 70
- Database :
- Academic Search Index
- Journal :
- Electronic Journal of Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 178376274
- Full Text :
- https://doi.org/10.1016/j.ejbt.2024.04.003