Back to Search
Start Over
Entamoeba moshkovskii as a potential model organism for Gal/GalNAc lectin intermediate subunit exhibition and functional identification.
- Source :
-
Drug Discoveries & Therapeutics . Jun2024, Vol. 18 Issue 3, p178-187. 10p. - Publication Year :
- 2024
-
Abstract
- In humans, Entamoeba histolytica is the main pathogen causing various amoebiases, while E. moshkovskii falls between being a pathogen and non-pathogen. The two species have similar behavior patterns but differ significantly in pathogenicity, with previous studies and clinical data indicating that E. moshkovskii has a low level of pathogenicity. Meaningfully, the biological characteristics of E. moshkovskii make it a potential model organism and a protein display platform for studying the functions of important Entamoeba proteins. Here, an Amoeba-pcDNA3.1 vector capable of overexpressing E. histolytica-sourced Igl-C protein was constructed and successfully transfected into E. moshkovskii. High levels of expression of the Igl-C, EGFP, and NeoR genes were identified in Igl-C-transfected trophozoites using qRT-PCR, and they were subsequently confirmed using immunoblotting. Transfection of Igl-C protein improved the adherence and phagocytosis of E. moshkovskii, demonstrating that E. histolytica Igl mediated amoebic adhesion. Moreover, as a manifestation of protein virulence, the ability of post-transfected trophozoites to induce inflammation in host macrophages was also enhanced. In conclusion, this study utilizing the characteristics of E. moshkovskii confirmed its potential to serve as a model organism. E. moshkovskii could replace E. histolytica as the target of gene editing, allowing more efficient study of amoebic pathogenicity. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENTAMOEBA
*ENTAMOEBA histolytica
*GENOME editing
*GENE targeting
*TROPHOZOITES
Subjects
Details
- Language :
- English
- ISSN :
- 18817831
- Volume :
- 18
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Drug Discoveries & Therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 178366325
- Full Text :
- https://doi.org/10.5582/ddt.2024.01031