Mechanism of antibacterial property of micro scale rough surface formed by fine-particle bombarding.

\nImpact statementFine-particle bombardment (FPB) is typically used to modify metal surfaces by bombarding them with fine particles at high speed. FPB is not a coating technique but is used for forming microscale concavities and convexities on a surface. Previously, we reported that an FPB-treated surface showed antibacterial effects; however, the underlying mechanisms remain unclear. We hypothesized that the pitch size of concavity and convexity, and irregular microscale pattern of FPB-treated surfaces might contribute to the antibacterial performance. In this study, we applied FPB to stainless-steel surfaces and evaluated the antibacterial effects of the FPB-treated surfaces based on ISO 22,196:2007. The FPB-treated surfaces exhibited antibacterial activity against <italic>Escherichia coli</italic>, with an antibacterial activity value (R) of two or more. Furthermore, our experiments suggest that the antibacterial mechanism of the FPB-treated surface can be attributed to increased oxidative stress in bacteria owing to physical stress from the rough surface. The antibacterial effect of FPB-treated surfaces offers an effective measure against drug-resistant bacteria.The antibacterial activity of FPB-treated surfaces can be attributed to increased oxidative stress induced by physical stress from the FPB-generated rough surface. [ABSTRACT FROM AUTHOR]