黄瓜和南瓜 Bcl-2 相关抗凋亡家族全基因组鉴定与表达 模式分析.

【Objective】In order to provide favorable genes for the analysis of the grafting healing mechanism of Cucumis sativus L. or Cucurbita moschata Duch. grafted seedlings and the molecular breeding of resistance of the cucumber and pumpkin vegetables, we conducted the analysis of the expression patterns of Bcl-2 associated athanogene（BAG）family proteins in the cucumber and pumpkin in response to abiotic stress and in response to light during cucumber or pumpkin grafting healing.【Method】Based on the genome information of the cucumber and pumpkin, the BAG gene family in the cucumber and pumpkin was identified through bioinformatics methods, and its physicochemical characteristics, chromosome mapping, gene structure, phylogeny and collinearity were analyzed. The expression patterns of BAG family proteins in the cucumber and pumpkin in response to abiotic stress and in response to light during cucumber or pumpkin grafting healing were analyzed. These data were based on the public database and the transcriptome sequencing data of the cucumber or pumpkin grafted seedlings in the process of grafting and healing.【Result】A total of 12 and 18 members of the BAG family were identified in the cucumber and pumpkin, respectively and they were all divided into two subfamilies and the gene members were highly conserved. The BAG of subfamily I was mainly involved in gene regulation and stress response, while the BAG of subfamily II was mainly involved in plant development. The BAG genes of cucumber and pumpkin had multiple linear relationships with Arabidopsis, Oryza sativa L. and Solanum lycopersicum L., respectively, but the CsaV3_1G017210 was relatively conservative and there was no collinearity with the BAG genes in Arabidopsis, rice, tomato and pumpkin. Different BAG genes possessed tissue-specific expression patterns. CsaV3_6G000970 and CmoCh08G008520 belonged to the BAG family molecular chaperone regulator 6. In both cucumber and pumpkin, they were upregulated during the grafting healing process and in response to abiotic stress.【Conclusion】BAG family genes respond differently to abiotic stress and light during the grafting healing process of the cucumber or pumpkin. These genes synergistically regulate the growth, development and grafting healing of the cucumber and pumpkin. BAGs play an important role in the process of the cucumber and pumpkin abiotic stress and cucumber or pumpkin grafting healing. [ABSTRACT FROM AUTHOR]