甲状腺乳头状癌组织微小 RNA-93-5p、微小 RNA-98-5p 表达与临床病理 特征和增殖、侵袭基因表达的关系研究.

Objective: To study the relationship between the expression of micro-93-5p (miR-93-5p) and microRNA-98-5p (miR-98-5p) in papillary thyroid carcinoma (PTC) and clinicopathological features, proliferation and invasion gene expression. Methods: 153 PTC patients who were underwent surgical resection in Guangdong Provincial Hospital of Traditional Chinese Medicine from October 2020 to October 2023 were selected as the research objects, and the intraoperatively resected cancer tissues and adjacent tissues were collected. The expression levels of miR-93-5p, miR-98-5p, proliferation gene and invasion gene mRNA in cancer tissues and adjacent tissues were detected and compared, and the relationship between the expression of miR-93-5p and miR-98-5p and the clinicopathological features of PTC patients were analyzed. The correlation between the expression levels of miR-93-5p and miR-98-5p and the mRNA expression of proliferation genes and invasion genes were analyzed by Pearson method. Results: The expression level of miR-93-5p in cancer tissues was higher than that in adjacent tissues, and the expression level of miR-98-5p was lower than that in adjacent tissues (P<0.05). The proportion of TNM stage III-IV, lymph node metastasis and poor differentiation in PTC patients with high expression of miR-93-5p were higher than those in PTC patients with low expression of miR-93-5p (P<0.05). The proportion of TNM stage III-IV, lymph node metastasis and poor differentiation in PTC patients with low expression of miR-98-5p were higher than those in PTC patients with high expression of miR-98-5p (P<0.05). The levels of proliferation gene programmed cell death factor 4 (PDCD4) and protein phosphatase 4 regulatory subunit (PP4R1) in cancer tissues were lower than those in adjacent tissues (P<0.05), and the levels of invasion gene metalloprotease disintegrin 9(ADAM9) and Bcl-6 co-inhibitor-like protein (BCORL1) were higher than those in adjacent tissues (P<0.05). Pearson analysis showed that, the expression of miR-93-5p was negatively correlated with the expression levels of proliferation genes PDCD4 and PP4R1, and positively correlated with the expression levels of invasion genes ADAM9 and BCORLI. The expression level of miR-98-5p was positively correlated with the levels of proliferation genes PDCD4 and PP4R1, and negatively correlated with the expression levels of invasion genes ADAM9 and BCORL1. Conclusion: The expression of miR-93-5p in cancer tissues of PTC patients is increase, and the expression of miR-98-5p is decrease, which are relate to clinicopathological features such as TNM stage, lymph node metastasis and differentiation degree, and can also promote the proliferation and invasion of PTC cancer cells. [ABSTRACT FROM AUTHOR]