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Alginate-gelatine hydrogel microspheres protect NK cell proliferation and cytotoxicity under hypoxic conditions.
- Source :
-
Journal of Microencapsulation . Aug2024, Vol. 41 Issue 5, p375-389. 15p. - Publication Year :
- 2024
-
Abstract
- This study aimed to encapsulate natural killer (NK) cells in a hydrogel to sustain their function within the hypoxic tumour microenvironments. An alginate-gelatine hydrogel was generated via electrospray technology. Hydrogel biocompatibility was assessed through cell counting kit-8 and Live/Dead assays to ascertain cell. Moreover, we analysed lactate dehydrogenase assays to evaluate the cytotoxicity against tumours and utilised RT-qPCR to analyse cytokine gene level. Alginate and gelatine formed hydrogels with diameters ranging from 489.2 ± 23.0 μm, and the encapsulation efficiency was 34.07 ± 1.76%. Encapsulated NK cells exhibited robust proliferation and tumour-killing capabilities under normoxia and hypoxia. Furthermore, encapsulation provided a protective shield against cell viability under hypoxia. Importantly, tumour-killing cytotoxicity through cytokines upregulation such as granzyme B and interferon-gamma was preserved under hypoxia. The encapsulation of NK cells not only safeguards their viability but also reinforces anticancer capacity, countering the inhibition of activation induced by hypoxia. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KILLER cells
*CYTOTOXINS
*HYDROGELS
*CELL proliferation
*ALGINATES
*MICROSPHERES
Subjects
Details
- Language :
- English
- ISSN :
- 02652048
- Volume :
- 41
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Microencapsulation
- Publication Type :
- Academic Journal
- Accession number :
- 178298732
- Full Text :
- https://doi.org/10.1080/02652048.2024.2362170