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SdrR, a LysR-type regulator, responds to the mycobacterial antioxidant defense.

Authors :
Zhu, Chen
Wei, Wen-ping
An, Jing-ning
Hu, Jia-ling
Gao, Chun-hui
Yang, Min
Source :
Journal of Biochemistry. Jul2024, Vol. 176 Issue 1, p43-54. 12p.
Publication Year :
2024

Abstract

Protection against oxidative stress is a vital defense mechanism for Mycobacterium tuberculosis within the host. However, few transcription factors that control bacterial antioxidant defense are known. Here, we present evidence that SdrR, encoded by the MSMEG_5712 (Ms5712) gene, functions as an oxidative stress response regulator in Mycobacterium smegmatis. SdrR recognizes an 11-bp motif sequence in the operon's upstream regulatory region and negatively regulates the expression of short-chain dehydrogenases/reductases (SDR). Overexpressing sdrR inhibited SDR expression, which rendered the strain oxidative more stress-sensitive. Conversely, sdrR knockout alleviates SDR repression, which increases its oxidative stress tolerance. Thus, SdrR responds to oxidative stress by negatively regulating sdr expression. Therefore, this study elucidated an underlying regulatory mechanism behind mycobacterial oxidative stress adaptation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0021924X
Volume :
176
Issue :
1
Database :
Academic Search Index
Journal :
Journal of Biochemistry
Publication Type :
Academic Journal
Accession number :
178237835
Full Text :
https://doi.org/10.1093/jb/mvae026