贵州野生刺梨叶黄酮的纯化工艺研究.

The purpose of this study is to extract total flavonoids from leaves of wild Rosa roxburghii Tratt. in Guizhou by ultrasonic extraction with 60% ethanol. The dynamic adsorption characteristics of five kinds of macroporous resins for total flavonoids were studied by static adsorption test, and the macroporous adsorption resins with good adsorption and desorption performance for flavonoids from Rosa roxburghii Tratt. leaves were screened out. The extraction process of flavonoids from Rosa roxburghii Tratt. leaves purified by macroporous resin was optimized with adsorption rate and desorption rate as indexes. The results showed that macroporous resin AB-8 had good adsorption and desorption performance. When the purification process was diameter-height ratio 1∶2 (2BV), the concentration of sample solution was 0.6 g/L, and the flow rate was 2 mL/min, AB-8 macroporous adsorption resin could quickly and effectively adsorb flavonoids from Rosa roxburghii Tratt. leaves. Flavonoids adsorbed by AB-8 macroporous adsorption resin could be quickly and fully eluted by using 55% ethanol 80 mL and maintaining an elution flow rate of 2 mL/min. Under these conditions, the purity of flavonoids from Rosa roxburghii Tratt. leaves increased from 3.368 mg/g to 6.679 mg/g. The study indicates that the technological conditions are suitable for the purification of flavonoids from Rosa roxburghii Tratt. leaves. [ABSTRACT FROM AUTHOR]