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Discovery of procyanidin condensed tannins of (−)-epicatechin from Kratom, Mitragyna speciosa, as virucidal agents against SARS-CoV-2.
- Source :
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International Journal of Biological Macromolecules . Jul2024:Part 1, Vol. 273, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
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Abstract
- Kratom, Mitragyna speciosa , is one of the most popular herbs in the West and Southeast Asia. A number of previous works have focused on bioactive alkaloids in this plant; however, non-alkaloids have never been investigated for their biological activities. Antiviral and virucidal assays of a methanol leaf extract of Kratom, M. speciosa , revealed that a crude extract displayed virucidal activity against the SARS-CoV-2. Activity-guided isolation of a methanol leaf extract of Kratom led to the identification of B-type procyanidin condensed tannins of (−)-epicatechin as virucidal compounds against SARS-CoV-2. The fraction containing condensed tannins exhibited virucidal activity with an EC 50 value of 8.38 μg/mL and a selectivity index (SI) value >23.86. LC-MS/MS analysis and MALDI-TOF MS identified the structure of the virucidal compounds in Kratom as B-type procyanidin condensed tannins, while gel permeation chromatograph (GPC) revealed weight average molecular weight of 238,946 Da for high molecular-weight condensed tannins. In addition to alkaloids, (−)-epicatechin was found as a major component in the leaves of M. speciosa , but it did not have virucidal activity. Macromolecules of (−)-epicatechin, i.e., procyanidin condensed tannins, showed potent virucidal activity against SARS-CoV-2, suggesting that the high molecular weights of these polyphenols are important for virucidal activity. • High amount of (−)-epicatechin was found in leaves of Kratom, Mitragyna speciosa. • Virucidal compounds in Kratom were identified as condensed tannins of (−)-epicatechin. • Procyanidin condensed tannins exhibited virucidal activity against COVID-19 virus with EC 50 value of 8.38 μg/mL. • This work first reports bioactive non-alkaloid compounds in Kratom. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 273
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 178210044
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2024.133059