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Establishment and Application of a Novel Genetic Detection Panel for SNPs in Mongolian Gerbils.

Authors :
Guo, Yafang
Cui, Yutong
Sun, Minghe
Zhu, Xiao
Zhang, Yilang
Lu, Jing
Li, Changlong
Lv, Jianyi
Guo, Meng
Liu, Xin
Chen, Zhenwen
Du, Xiaoyan
Huo, Xueyun
Source :
Genes. Jun2024, Vol. 15 Issue 6, p817. 11p.
Publication Year :
2024

Abstract

The Mongolian gerbil is a distinctive experimental animal in China, as its genetic qualities possess significant value in the field of medical biology research. Here, we aimed to establish an economical and efficient panel for genetic quality detection in Mongolian gerbils using single-nucleotide polymorphism (SNP) markers. To search for SNPs, we conducted whole-genome sequencing (WGS) in 40 Mongolian gerbils from outbred populations. Reliable screening criteria were established to preliminarily select SNPs with a wide genome distribution and high levels of polymorphism. Subsequently, a multiple-target regional capture detection system based on second-generation sequencing was developed for SNP genotyping. Based on the results of WGS, 219 SNPs were preliminarily selected, and they were established and optimized in a multiple-amplification system that included 206 SNP loci by genotyping three outbred populations. PopGen.32 analysis revealed that the average effective allele number, Shannon index, observed heterozygosity, expected heterozygosity, average heterozygosity, polymorphism information content, and other population genetic parameters of the Capital Medical University (CMU) gerbils were the highest, followed by those of Zhejiang gerbils and Dalian gerbils. Through scientific screening and optimization, we successfully established a novel, robust, and cost-effective genetic detection system for Mongolian gerbils by utilizing SNP markers for the first time. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20734425
Volume :
15
Issue :
6
Database :
Academic Search Index
Journal :
Genes
Publication Type :
Academic Journal
Accession number :
178192542
Full Text :
https://doi.org/10.3390/genes15060817