Desarrollo y multiplicación in vitro de orquídea Brassolaeliocattleya.

In vitro propagation is an important technique to produce orchids since it allows the multiplication and marketing of specimens with outstanding characteristics that have more commercial acceptance. By following this practice, natural populations are protected and the use of orchids is carried out in a sustainable manner. The objective was to evaluate the effect of three concentrations of 6-benziladenine (BA) on the growth and development of protocorms in cattleya (Brassolaeliocattleya) seedlings in vitro. The protocorms from the seeds were subcultured to a new medium with the same characteristics but adding BA in three different treatments: 1.0 mg·L-1, 1.5 mg·L-1 and 2.0 mg·L-1. The in vitro growth and development of protocorms presented a better result by adding BA in 1.5 mg L-1 doses, where plant height, leaf length, leaf number and root length were promoted, obtaining large and vigorous seedlings for transplanting and acclimatization. [ABSTRACT FROM AUTHOR]