Unveiling the anti-vitiligo, anti-inflammatory, and antitumor activities of sesquiterpene coumarins isolated from Ferula kuhistanica.

Six undescribed bicyclic sesquiterpene coumarins, kuhistanin A, ferukrin isovalerate, 9' β ,12' α - ferukrin isovalerate, (17′ E)- 9' α , 12' β - isomarcandin, (17′ Z)- 9' α , 12' β - isomarcandin and (17′ E) - isomarcandin, together with nine known ones were isolated from the roots of Ferula kuhistanica Korovin. The structures of them were elucidated using NMR and HRESIMS data analysis. The relative configurations of the isolates were confirmed by NOE correlations and NMR calculation. The absolute configurations of them were confirmed by X-ray diffraction analysis and ECD calculation. Anti-vitiligo, anti-inflammatory and cytotoxicity of the isolates were tested. Acetyl feselol, feselol, ferusingensine I and farnesiferol A significantly increased the melanin content at the concentration of 10 μM. (17′ E) - 9' α , 12' β - isomarcandin exhibited strong cytotoxicity against HT-29 cell line with IC 50 values of 8.94 ± 0.47 μM, and (17′ E) - isomarcandin demonstrated strong cytotoxicity against Hela, A549 and HT-29 cell lines with IC 50 values of 5.29 ± 0.25, 4.01 ± 0.20, and 4.16 ± 0.21 μM, respectively. This study concluded that, isolated compounds from F. kuhistanica demonstrated strong bioactivity towards anti-vitiligo and cytotoxicity and active compounds are suggested as anti-vitiligo and cytotoxicity agent for future drug development. Six undescribed sesquiterpene coumarins kuhistanin A, ferukrin isovalerate, 9' β ,12' α - ferukrin isovalerate, (17′ E)- 9' α , 12' β - isomarcandin, (17' Z)- 9' α , 12' β - isomarcandin and (17′ E) - isomarcandin, together with nine known ones were isolated from the roots of F. kuhistanica. The isolates were tested their anti-vitiligo, anti-inflammatory and cytotoxic activities. [Display omitted] • Six undescribed sesquiterpene coumarins were isolated from F. kuhistanica roots • The structural elucidation of these compounds was described. • Anti-vitiligo and cytotoxic activities of the isolates were tested. [ABSTRACT FROM AUTHOR]