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Unique features of conventional and nonconventional introns in Euglena gracilis.
- Source :
-
BMC Genomics . 6/13/2024, Vol. 25 Issue 1, p1-10. 10p. - Publication Year :
- 2024
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Abstract
- Background: Nuclear introns in Euglenida have been understudied. This study aimed to investigate nuclear introns in Euglenida by identifying a large number of introns in Euglena gracilis (E. gracilis), including cis-spliced conventional and nonconventional introns, as well as trans-spliced outrons. We also examined the sequence characteristics of these introns. Results: A total of 28,337 introns and 11,921 outrons were identified. Conventional and nonconventional introns have distinct splice site features; the former harbour canonical GT/C-AG splice sites, whereas the latter are capable of forming structured motifs with their terminal sequences. We observed that short introns had a preference for canonical GT-AG introns. Notably, conventional introns and outrons in E. gracilis exhibited a distinct cytidine-rich polypyrimidine tract, in contrast to the thymidine-rich tracts observed in other organisms. Furthermore, the SL-RNAs in E. gracilis, as well as in other trans-splicing species, can form a recently discovered motif called the extended U6/5' ss duplex with the respective U6s. We also describe a novel type of alternative splicing pattern in E. gracilis. The tandem repeat sequences of introns in this protist were determined, and their contents were comparable to those in humans. Conclusions: Our findings highlight the unique features of E. gracilis introns and provide insights into the splicing mechanism of these introns, as well as the genomics and evolution of Euglenida. [ABSTRACT FROM AUTHOR]
- Subjects :
- *TANDEM repeats
*EUGLENA gracilis
*INTRONS
*ALTERNATIVE RNA splicing
Subjects
Details
- Language :
- English
- ISSN :
- 14712164
- Volume :
- 25
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BMC Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 177884668
- Full Text :
- https://doi.org/10.1186/s12864-024-10495-9