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Mutation breeding of high-stress resistant strains for succinic acid production from corn straw.
- Source :
-
Applied Microbiology & Biotechnology . 2/1/2024, Vol. 108 Issue 1, p1-11. 11p. - Publication Year :
- 2024
-
Abstract
- The production of succinic acid from corn stover is a promising and sustainable route; however, during the pretreatment stage, byproducts such as organic acids, furan-based compounds, and phenolic compounds generated from corn stover inhibit the microbial fermentation process. Selecting strains that are resistant to stress and utilizing nondetoxified corn stover hydrolysate as a feedstock for succinic acid production could be effective. In this study, A. succinogenes CICC11014 was selected as the original strain, and the stress-resistant strain A. succinogenes M4 was obtained by atmospheric and room temperature plasma (ARTP) mutagenesis and further screening. Compared to the original strain, A. succinogenes M4 exhibited a twofold increase in stress resistance and a 113% increase in succinic acid production when hydrolysate was used as the substrate. By conducting whole-genome resequencing of A. succinogenes M4 and comparing it with the original strain, four nonsynonymous gene mutations and two upstream regions with base losses were identified. Key points: • A high-stress-resistant strain A. succinogenes M4 was obtained by ARTP mutation • The production of succinic acid increased by 113% • The mutated genes of A. succinogenes M4 were detected and analyzed [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01757598
- Volume :
- 108
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Applied Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 177878109
- Full Text :
- https://doi.org/10.1007/s00253-024-13112-7