Purification, identification and molecular docking studies of antioxidant and anti-inflammatory peptides from Edible Bird's Nest.

This study investigated antioxidant and anti-inflammatory peptides from Edible Bird's Nest (EBN). The prepared EBN peptides were sequentially separated, purified, and successively identified by ultrafiltration, gel filtration and mass spectrometry techniques. Four potential antioxidant and anti-inflammatory peptides were identified as Peptide 1 (LFWSPSVYLK), Peptide 2 (GWPHLEDNYLDW), Peptide 3 (NPPADLHK) and Peptide 4 (GDLAYLDQGHR). Molecular docking analysis revealed that Peptide 1 and Peptide 2 can competitively interrupt the formation of Keap1-Nrf2 due to the presence of hydrophobic and antioxidant amino acids in their peptide sequences. Peptide 3 and Peptide 4 have a strong effect on interacting with the binding site of IKK-β due to the interaction of anti-inflammatory amino acids and C -terminal arginine/lysine. The four peptides were synthesised and validated for their antioxidant and anti-inflammatory activities. The results suggest that the four peptides may serve as promising bioactive peptides for preventing oxidative stress and inflammation-related diseases. [Display omitted] • Edible Bird's Nest (EBN) peptides were successfully separated and purified. • Four potent antioxidant and anti-inflammatory peptides were identified. • Interaction between peptides in the Keap1-Nrf2 and IKK-β pathways was investigated. • Peptides (LFWSPSVYLK and GWPHLEDNYLDW) showed high antioxidant capacity. • Peptides (NPPADLHK and GDLAYLDQGHR) showed high anti-inflammatory capacity. [ABSTRACT FROM AUTHOR]