Pyrazole probes for the detection of N2H4 with ICT properties in live cells and soils.

• The two novel fluorescent probes Z 1 and Z 2 based have been designed and synthesized base on pyrazole derivative to recognize hydrazine • The probe Z 1-2 have ICT characteristics with large Stokes shifts. • The probe Z 1-2 could be made into test paper strips for detection of hydrazine. • The probe Z 1-2 can be used in detection of N 2 H 4 in environmental soil samples and cell imaging. Here, the two novel fluorescent probes Z 1 and Z 2 have been designed and synthesized based on pyrazole derivative to recognize hydrazine, and the molecular structure was determined by 1H NMR, 13C NMR, and ESI-MS. The obtained probe molecules have AIE and ICT characteristics. Nuclear magnetic resonance analysis (NMR) and theoretical calculations were used to confirm the identification mechanism. Probes Z 1 and Z 2 have good selectivity, low detection limits (0.197 µM and 0.185 µM, respectively), and high sensitivity. Probes Z 1 and Z 2 have large Stokes shifts of 198 nm and 211 nm, respectively. The probes also could be made into test paper strips for the detection of hydrazine. Furthermore, the probes can be used in the detection of N 2 H 4 in environmental soil samples and cell imaging. The results showed that the synthetic probe had good biocompatibility. Cell imaging experiments were performed to apply probes to the detection of hydrazine hydrate in HeLa cells. [Display omitted] [ABSTRACT FROM AUTHOR]