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Insights into conformational changes in cytochrome b during the early steps of its maturation.

Authors :
Carlström, Andreas
Ott, Martin
Source :
FEBS Letters. Jun2024, Vol. 598 Issue 11, p1438-1448. 11p.
Publication Year :
2024

Abstract

Membrane proteins carrying redox cofactors are key subunits of respiratory chain complexes, yet the exact path of their folding and maturation remains poorly understood. Here, using cryo‐EM and structure prediction via Alphafold2, we generated models of early assembly intermediates of cytochrome b (Cytb), a central subunit of complex III. The predicted structure of the first assembly intermediate suggests how the binding of Cytb to the assembly factor Cbp3‐Cbp6 imposes an open configuration to facilitate the acquisition of its heme cofactors. Moreover, structure predictions of the second intermediate indicate how hemes get stabilized by binding of the assembly factor Cbp4, with a concomitant weakening of the contact between Cbp3‐Cbp6 and Cytb, preparing for the release of the fully hemylated protein from the assembly factors. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00145793
Volume :
598
Issue :
11
Database :
Academic Search Index
Journal :
FEBS Letters
Publication Type :
Academic Journal
Accession number :
177798896
Full Text :
https://doi.org/10.1002/1873-3468.14888