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Antigen-level resolution of commensal-specific B cell responses can be enabled by phage display screening coupled with B cell tetramers.
- Source :
-
Immunity (10747613) . Jun2024, Vol. 57 Issue 6, p1428-1428. 1p. - Publication Year :
- 2024
-
Abstract
- Induction of commensal-specific immunity contributes to tissue homeostasis, yet the mechanisms underlying induction of commensal-specific B cells remain poorly understood in part due to a lack of tools to identify these cells. Using phage display, we identified segmented filamentous bacteria (SFB) antigens targeted by serum and intestinal antibodies and generated B cell tetramers to track SFB-specific B cells in gut-associated lymphoid tissues. We revealed a compartmentalized response in SFB-specific B cell activation, with a gradient of immunoglobulin A (IgA), IgG1, and IgG2b isotype production along Peyer's patches contrasted by selective production of IgG2b within mesenteric lymph nodes. V(D)J sequencing and monoclonal antibody generation identified somatic hypermutation driven affinity maturation to SFB antigens under homeostatic conditions. Combining phage display and B cell tetramers will enable investigation of the ontogeny and function of commensal-specific B cell responses in tissue immunity, inflammation, and repair. [Display omitted] • Phage display screening identifies immunogenic commensal antigens • B cell antigen tetramers enable identification of SFB-specific B cells • SFB-specific B cells display anatomically compartmentalized effector functions • Affinity maturation of SFB-specific B cells occurs under homeostatic conditions Adaptive immunity to commensal microbes promotes tissue homeostasis, immunity, and repair, but approaches to identify commensal-specific B cell responses are lacking. Verma et al. utilize phage display screening and subsequent generation of B cell tetramers to identify anatomical compartmentalization of SFB-specific B cell responses in gut-associated lymphoid tissues. [ABSTRACT FROM AUTHOR]
- Subjects :
- *B cells
*HOMEOSTASIS
*LYMPHOID tissue
*IMMUNE response
*FILAMENTOUS bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 10747613
- Volume :
- 57
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Immunity (10747613)
- Publication Type :
- Academic Journal
- Accession number :
- 177747291
- Full Text :
- https://doi.org/10.1016/j.immuni.2024.04.014