基于BSA-seq 技术挖掘番茄耐裂果候选基因.

[Objective] To determine the position of tomato crack tolerance gene on chromosome. [Method] Tomato 14FP5 which is resistant to cracking and tomato 14FP26 which is easy to crack are used as test materials, cross the two to get F1 generation, F1 self-cross to get F2, DNA mixing pools with two extreme traits were constructed respectively, BSA-seq method was used to locate the location. [Result] BSA-seq was used for genome resequencing analysis of 2 extreme mixed pools of F2 population. The tomato anti-cracking gene was initially located on chromosome 5, located between 34 140 000 and 38 120 000 pb, the interval size is 3.98 Mb, a total of 38 genes were annotated in the candidate region, including 4 non-synonymous mutant genes. [Conclusion The results provide data support for the fine localization of tomato crack tolerance genes, and lay a foundation for tomato crack tolerance molecular breeding. [ABSTRACT FROM AUTHOR]