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Star1 gene mutation reveals the essentiality of 11-ketotestosterone and glucocorticoids for male fertility in Nile Tilapia (Oreochromis niloticus).
- Source :
-
Comparative Biochemistry & Physiology - Part B: Biochemistry & Molecular Biology . Aug2024, Vol. 273, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
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Abstract
- Steroidogenic acute regulatory protein (Star) plays an essential role in the biosynthesis of corticosteroids and sex steroids by mediating the transport of cholesterol from the outer to the inner membrane of mitochondria. Two duplicated Star genes, namely star1 and star2 , have been identified in non-mammalian vertebrates. To investigate the roles of star genes in fish steriodogenesis, we generated two mutation lines of star1 −/− and star1 −/− /star2 −/− in Nile tilapia (Oreochromis niloticus). Previous studies revealed that deficiency of star2 gene caused delayed spermatogenesis, sperm apoptosis and sterility in male tilapia. Our present data revealed that mutation of star genes impaired male fertility. Disordered seminiferous lobules and spermatic duct obstruction were found in the testis of both types of mutants. Moreover, significant decline in semen volume, sperm abnormality and impaired fertility were also detected in star1 −/− and star1 −/− /star2 −/− males. In star1 −/− male fish, lipid accumulation, up-regulation of steroidogenic enzymes, and significant decline of androgens were found. Additionally, hyperplasic interrenal cells, elevated steroidogenic gene expression level and decline of serum glucocorticoids were detected in star1 mutants. Intriguingly, either 11-KT or cortisol supplementation successfully rescued the impaired fertility of the star1 −/− mutants. Taken together, these results further indicate that Star1 might play critical roles in the production of both 11-KT and glucocorticoids, which are indispensable for the maintenance of male fertility in fish. [Display omitted] • Star1 plays critical roles in androgen and glucocorticoids synthesis in tilapia. • Star1 and star1 / star2 gene mutation impaired male fertility in tilapia. • 11-keto-testosterone and cortisol can restore male fertility in star1 mutant tilapia. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10964959
- Volume :
- 273
- Database :
- Academic Search Index
- Journal :
- Comparative Biochemistry & Physiology - Part B: Biochemistry & Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 177601368
- Full Text :
- https://doi.org/10.1016/j.cbpb.2024.110985