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Multi-target aptamer assay for endocrine-disrupting phthalic acid ester panel screening in plastic leachates.
- Source :
-
Chemosphere . Jul2024, Vol. 359, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- A multi-target aptamer assay was developed as a phthalic acid ester (PAE) panel to screen selected PAEs in plastic leachate samples. The panel comprises 13 PAEs (PAE-13), namely dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate, di-n-hexyl phthalate, diisobutyl phthalate, diisononyl phthalate, diisodecyl phthalate, mono-2-ethylhexyl phthalate, di-2-ethylhexyl phthalate, diphenyl phthalate, butyl benzyl phthalate, dicyclohexyl phthalate, and phthalic acid. Herein, we proposed an aptamer assay using a newly truncated aptamer (20-mer) and the 7-aminoactinomycin D fluorophore, which selectively binds to guanine in single-stranded DNA, resulting in increased fluorescence intensity. The assay is highly selective for PAE-13 clusters. The selectivity of the assay was evaluated using 13 different PAEs and mixtures depending on the side chain structure. The quantitative detection of PAEs was demonstrated by adopting mixed PAE-13 simulants and achieved a limit of detection of ∼1.4 pg/mL. The repeatability and reproducibility of the assay were also evaluated by presenting acceptable coefficients of variation (%CV less than 10% and 15%, respectively). The performance of the assay was demonstrated by analyzing the plastic leachate samples, and the positive correlation (correlation coefficient, r = 0.985) was confirmed by comparing them with the total sum of individual PAE peak areas obtained by gas chromatography mass spectrometry analysis. Development of a multi-target aptamer assay for endocrine disrupting phthalic acid esters panel screening in plastic leachates. [Display omitted] • Fluorescence signal-based multi-target aptamer assay for PAE panel (PAE-13) screening. • Truncated aptamer design with 20-mer length improving PAE-13 screening. • Quantifying PAE-13 with statistically acceptable repeatability and reproducibility. • Highly sensitive detection with LOD ∼1.4 pg/mL (ppt) for PAE-13 screening. • Total PAEs in plastic leachates showing the strong correlation (r = 0.985) with GC-MS analysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00456535
- Volume :
- 359
- Database :
- Academic Search Index
- Journal :
- Chemosphere
- Publication Type :
- Academic Journal
- Accession number :
- 177565790
- Full Text :
- https://doi.org/10.1016/j.chemosphere.2024.142366