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蛋白质的非定点荧光标记 --推荐一个化学生物学实验.
- Source :
-
University Chemistry . 2024, Vol. 39 Issue 4, p351-360. 10p. - Publication Year :
- 2024
-
Abstract
- This paper presents a chemical biology experiment designed to exploit the principle of covalent binding between fluorescein isothiocyanate and protein amines. This process is aimed at achieving non-site-specific fluorescent labeling of both extracellular proteins, exemplified by bovine serum albumin, and intracellular proteins, using Escherichia coli total protein as a case study. Gel filtration chromatography is employed as a subsequent purification method, with comprehensive characterization of the labeling results performed using UV-Visible spectrophotometry, flow cytometry, and fluorescence microscopy. This experiment encompasses a range of foundational and cutting-edge laboratory skills. It is closely aligned with the commonly employed antibody fluorescence labeling techniques in scientific research, presenting a moderate level of complexity. Participation in this experiment allows students to gain a profound understanding of fundamental concepts in chemical biology, enhance their comprehensive practical skills, broaden their research perspectives and nurture scientific thinking. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 10008438
- Volume :
- 39
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- University Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 177543875
- Full Text :
- https://doi.org/10.3866/PKU.DXHX202310010