The added value of automated HPC count: detecting clinically important interferences on the flow cytometric CD34+ cell count.

This letter discusses the importance of accurately and efficiently measuring CD34+ stem cell counts in peripheral blood for hematopoietic stem cell transplantation (HSCT). The authors describe a case in which there was a discrepancy between the results of the hematopoietic progenitor cell (HPC) count and the flow cytometric CD34+ cell count, indicating interference in the flow cytometric analysis. They conducted alternative staining methods and washing techniques to resolve the issue and ultimately confirmed the presence of CD34+ stem cells. The authors emphasize the value of HPC count as a quality control parameter and highlight the importance of careful review and communication in patient treatment. [Extracted from the article]