Back to Search
Start Over
Determination of Cholesterol by Colorimetry and Surface-Enhanced Raman Spectroscopy Using Gold Nanoparticles Decorated with a Fe(III)-Tetra(4-Carboxyphenyl)-Porphine Metal-Organic Framework.
- Source :
-
Analytical Letters . 2024, Vol. 57 Issue 12, p1861-1875. 15p. - Publication Year :
- 2024
-
Abstract
- A simple and facile hydrothermal method was developed for the synthesis of gold nanoparticles (AuNPs) adorned with two-dimensional (2D) heme-like Fe(III)-tetra(4-carboxyphenyl)-porphine (TCPP-Fe) metal-organic framework (MOF) nanocomposite. Notably, the synthesized hybrid nanomaterial (AuNPs/TCPP-Fe hybrid nanosheets) exhibited excellent peroxidase (POD)-like nanoenzymes activity and Raman activity under ultrasound (US) and near-infrared (NIR) irradiation at physiological pH conditions. A cascade colorimetric-SERS dual-mode method was developed for sensitive and rapid cholesterol determination based on the excellent POD-like properties and surface-enhanced Raman spectroscopy (SERS) activity of AuNPs/TCPP-Fe nanoenzymes. The TCPP-Fe nanoenzymes combined with cholesterol oxidase catalyze a cascade reaction to convert cholesterol to H2O2, which then oxidizes colorless, low Raman-activity 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue, high-Raman-activity oxidation product (ox-TMB). The cholesterol in human serum of colorimetric-SERS was successfully assayed with good recovery from 97.8 to 103.7%. The study paves a new method for designing a nanocomposite-based SERS for biochemistry analysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032719
- Volume :
- 57
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Analytical Letters
- Publication Type :
- Academic Journal
- Accession number :
- 177520657
- Full Text :
- https://doi.org/10.1080/00032719.2023.2279251