不同饲料蛋白质水平对克氏原螯虾生长、 肌肉成分、消化酶活性、抗氧化能力的影响.

This experiment was conducted to investigate the effects of dietary protein levels on growth performance, muscle composition, digestive enzyme activity and antioxidant capacity of red swamp crayfish (Procambarus clarkii). Fish meal, soybean meal and rapeseed meal were the main sources of protein, soybean oil was the main source of fat, and flour was the main source of carbohydrate. The isoenergetic diets with 27.58%, 32.26% and 36.03% protein levels were prepared and named D1, D2 and D3 respectively. The crayfish with a mean initial body weight of (6.0±0.5) g were fed for 5 weeks, and three aquariums were fed with each experimental crayfish. The results showed that the final body weight and weight gain rate of crayfish increased with the increase of dietary protein level, and the differences were significant among the groups (P<0.05). There was no significant effect of different protein level feeds on muscle composition of crayfish (P>0.05). The activity of crayfish protease in each treatment group was significantly different (P<0.05), and the activity of protease in 36.03% protein diet reached the highest level, which was 46.57 U/mg. At the same time, the lipase activity in hepatopancreas was stimulated by high protein diet, and the highest level was detected in the D3, which was significantly different from that of D1(27.58%) and D2(32.26%) groups (P<0.05). CAT activity in D3 group was significantly higher than that in other two groups (P<0.05), and MAD activity in D1 group was significantly higher than that in other two groups (P<0.05). To sum up, suitably increasing the protein level in feed can promote the growth of crayfish and enhance their immunity to a certain extent. [ABSTRACT FROM AUTHOR]