Back to Search Start Over

Supramolecular citrate poly allylamine hydrochloride nanoparticles for citrate delivery and calcium oxalate nanocrystal dissolution.

Authors :
Gianvincenzo, Paolo Di
Leyes, Marcos Fernandez
Boonkam, Kamonchat
Puentes, Alejandro Fábrega
Reyes, Santiago Gimenez
Nardi, Alessandro Nicola
Olivieri, Alessio
Pummarin, Siwanut
Kamonsutthipaijit, Nuntaporn
Amenitsch, Heinz
Ritacco, Hernan
D'Abramo, Marco
Ortore, Maria Grazia
Boonla, Chanchai
Moya, Sergio E.
Source :
Journal of Colloid & Interface Science. Sep2024, Vol. 669, p667-678. 12p.
Publication Year :
2024

Abstract

[Display omitted] Renal calculi (kidney stones) are mainly made by calcium oxalate and can cause different complications including malfunction of the kidney. The most important urinary stone inhibitors are citrate molecules. Unfortunately, the amount of citrate reaching the kidney after oral ingestion is low. We hypothesized that nanoparticles of polyallylamine hydrochloride (CIT-PAH) carrying citrate ions could simultaneously deliver citrates while PAH would complex oxalate triggering dissolution and removal of CaOx nanocrystals. We successfully prepared nanoparticles of citrate ions with polyallylamine hydrochloride (CIT-PAH), PAH with oxalate (OX-PAH) and characterize them by Small Angle X ray Scattering (SAXS), Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS) and NMR. Dissolution of CaOx nanocrystals in presence of CIT-PAH have been followed with Wide Angle Xray Scattering (WAXS), DLS and Confocal Raman Microscopy. Raman spectroscopy was used to study the dissolution of crystals in synthetic urine samples. The release of citrate from CIT-PAH was followed by diffusion NMR. Molecular dynamics (MD) simulations were carried out to study the interaction of CIT and OX ions with PAH. CIT-PAH nanoparticles dissolves CaOx nanocrystals as shown by NMR, DLS, TEM and WAXS in water and by Raman spectroscopy in artificial human urine. WAXS and Raman show that the crystal structure of CaOx disappears in the presence of CIT-PAH. DLS shows that the time required for CaOX dissolution will depend on the concentration of CIT-PAH NPs. NMR proves that citrate ions are released from the CIT PAH NPs during CaOX dissolution, MD simulations showed that oxalates exhibit a stronger interaction for PAH than citrate, explaining the removal of oxalate ions and replacement of the citrate in the polymer nanoparticles. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219797
Volume :
669
Database :
Academic Search Index
Journal :
Journal of Colloid & Interface Science
Publication Type :
Academic Journal
Accession number :
177420512
Full Text :
https://doi.org/10.1016/j.jcis.2024.04.185