Two previously undescribed cholestanol saponins from the rhizomes of Paris fargesii var. petiolata.

Two previously undescribed cholestanol saponins, parpetiosides F − G (1 – 2), and six known analogs (3 – 8) were isolated from the rhizomes of Paris fargesii var. petiolata. Their structures were elucidated by extensive spectroscopic data analysis and chemical methods. Compound 1 was a rare 6/6/6/5/5 fused-rings cholestanol saponin with disaccharide moiety linked at C-26 of aglycone which was hardly seen in genus Paris. All of these compounds were discovered in this plant for the first time. In addition, the cytotoxicities of saponins (1 – 8) against three human cancer cell lines (U87, HepG2 and SGC-7901) were evaluated by CCK-8 method, and saponins 5 – 8 displayed certain cytotoxicities. The strong interactions between saponins 5 – 8 and SCUBE3, an oncogene for glioma cells, were displayed by molecular docking. [Display omitted] • Four cholestanol saponins were characterized from Paris fargesii var. petiolata , including two previously underscribed saponins (1–2). • Compound 1 was a rare 6/6/6/5/5 fused-rings cholestanol saponin with a rare disaccharide moiety linked at C-26 of aglycone. • The cytotoxicities of obtained compounds 1– 8 were evaluated. • The docking interactions between saponins 5 – 8 and SCUBE3 were described. [ABSTRACT FROM AUTHOR]