Fabrication and characterization of polydopamine-mediated zein-based nanoparticle for delivery of bioactive molecules.

In this study, a combination of whey protein (hydrophilic coating) and polydopamine (crosslinking agent) was used to improve the stability and functionality of quercetin-loaded zein nanoparticles. There are two key benefits of the core-shell nanoparticles formed. First, the ability of the polydopamine to bind to both zein and whey protein facilitates the formation of a stable core-shell structure, thereby protecting quercetin from any pro-oxidants in the aqueous surroundings. Second, neutral and hydrophilic whey proteins were used for the surface coating of the nanoparticles to further enhance the sustained and slow release of quercetin, facilitating its sustained release into the body at a slow and steady rate. The results of this study will promote the innovative development of precise nutritional delivery systems for zein and provide a theoretical basis for the design and development of dietary supplements based on hydrophobic food nutrient molecules. [Display omitted] • Mussel-inspired polydopamine-mediated zein/whey protein nanoparticle was designed. • Polydopamine-induced zein-based NPs protected QUE against environment stress. • The zein-based NPs improved antioxidant and antimicrobial activity of QUE. • The study offers new insights into the design of novel polyphenols delivery system. [ABSTRACT FROM AUTHOR]