人类辅助生殖技术受孕的稽留流产患者绒毛 组织染色体异常结果分析.

Objective To analyze the chromosomal abnormalities in villus tissue of missed abortion patients with different assisted reproductive techniques.Methods A total of 260 missed abortion patients admitted to the hospital from July 2019 to July 2023 who received assisted conception were selected as the assisted conception group.According to the different assisted reproductive techniques, they were divided into the in-vitro fertilization (IVF) group (135 cases),the artificial fertilization (AIH) group (45 cases) and the intracytoplasmic sperm injection (ICSI) group (80 cases). And a total of 42 missed abortion patients with natural conception were selected as the control group, and the chorionic tissue of the patients in each group was collected.The chromosome copy number variation (CNV) and abnormal number of chromosomes were observed by high-throughput sequencing.Results There was no significant difference in chromosome abnormality detection rate, CNV abnormality rate and number abnormality type between the assisted conception group and the control group (P＞0.05). There was no significant difference in chromosome abnormality detection rate, CNV abnormality rate and number abnormality type in the IVF group compared with the AIH group and the ICSI group (P＞0.05). In the assisted conception group, 16/22/21-trisomy abnormalities were predominant in the patients with abnormal chromosome numbers, while ditrisomy abnormalities were predominant in 48,XX+3,+18,48,XXY,+21,and X monomer abnormalities were all 45,X.Among the patients with triploid abnormalities, five patients were 69,XXY and two patients were 69,XXX.There was no significant difference in the distribution of abnormal karyotypes among all the groups (P＞0.05). Conclusion Assisted reproductive technology does not increase the risk of chromosomal abnormalities, and the distribution of abnormal chromosome karyotypes has nothing to do with the assisted reproductive technology method, and the assisted conception method can be selected according to the overall situation of the patient. [ABSTRACT FROM AUTHOR]