A combined lipidomic and proteomic profiling of Arabidopsis thaliana plasma membrane.

SUMMARY: The plant plasma membrane (PM) plays a key role in perception of environmental signals, and set‐up of adaptive responses. An exhaustive and quantitative description of the whole set of lipids and proteins constituting the PM is necessary to understand how these components allow to fulfill such essential physiological functions. Here we provide by state‐of‐the‐art approaches the first combined reference of the plant PM lipidome and proteome from Arabidopsis thaliana suspension cell culture. We identified and quantified a reproducible core set of 2165 proteins, which is by far the largest set of available data concerning this plant PM proteome. Using the same samples, combined lipidomic approaches, allowing the identification and quantification of an unprecedented repertoire of 414 molecular species of lipids showed that sterols, phospholipids, and sphingolipids are present in similar proportions in the plant PM. Within each lipid class, the precise amount of each lipid family and the relative proportion of each molecular species were further determined, allowing to establish the complete lipidome of Arabidopsis PM, and highlighting specific characteristics of the different molecular species of lipids. Results obtained point to a finely tuned adjustment of the molecular characteristics of lipids and proteins. More than a hundred proteins related to lipid metabolism, transport, or signaling have been identified and put in perspective of the lipids with which they are associated. This set of data represents an innovative resource to guide further research relative to the organization and functions of the plant PM. Significance Statement: In this study, we provide by state‐of‐the‐art approaches a combined reference of the plant plasma membrane (PM) lipidome and proteome from Arabidopsis thaliana suspension cell culture, identifying 414 lipids and 2165 proteins. This dataset, including a detailed analysis of the characteristics of lipids and proteins identified, provides a valuable resource to better explore and understand the organization and functional properties of the plant PM. [ABSTRACT FROM AUTHOR]