COL1A1 和COL1A2 基因生信分析及其在梅花鹿不同组织中的表达谱.

(Objective] The present paper aimed to explore the expression profiles of COL1A1 (Collagen type I alpha 1 chain) and COL1A2 (Collagen type I alpha 2 chain) genes in different tissues of sika deer, analyze their effects on tissue development of sika deer, and provide a basis for selecting candidate genes affecting important economic traits of sika deer. (Method) The expression levels of COL1A1 and COL1A2 genes in heart, liver, spleen, lung, kidney, large intestine, small intestine, cecum, duodenum, rumen, reticulum, omasum, abomasum, longissimus dorsi, leg muscle and testis of male sika deer were detected by RT-qPCR. The biological information of COL1A1 and COL1A2 genes and their expression profiles in different tissues of sika deer were predicted and analyzed by NetPhos 3.1, Motif Search and ProtParam software. On the basis, the phylogenetic tree of COL1A1 and COL1 A2 amino acid sequences was constructed. [Result] CDS regions of COL1AI and COL1A2 genes encoded 1463 and 1364 amino acids, respectively, and the theoretical PI were 5.60 and 9. 19, respecсtively. Both COL1A1 and COL1 A2 were hydrophilic and stable proteins with signal peptides and phosphorylation sites. The secondary and tertiary structures of COL1A1 and COL1A2 proteins were composed of random coils. Compared with other animals, deer COL1A1 and COL1A2 genes had the highest homology and the closest relationship with ruminant goats, sheep and cattle. The homology of COL1AI of deer with goats, cattle and sheep was 99.5%, 99.5% and 99.2%, respectively. The homology of COL1A2 of deer with goats, cattle and sheep was 99.1%, 99.0% and 98.9%, respectively. The results of RT-qPCR showed that COL1A1 and COL1A2 genes were expressed in different tissues of sika deer. The expression of COL1A1 gene was higher in heart, longissimus dorsi and leg muscle, which was significantly higher than that in other tissues, while the expression of COL1A2 gene was higher in heart, liver, kidney and omasum, which was significantly higher than that in other tissues. In addition, the expression of COL1A1 was higher in muscle tissue, while the expression of COL1A2 was lower. The expression of COL1A1 and COL1A2 in other tissues had a trend of high and low and synergistic effect. [Conclusion] COL1A1 and COL1A2 may cooperatively maintain tissue structure and tissue development, which lays a foundation for further research on the effects of COL1A1 and COL1A2 on growth and development of sika deer. [ABSTRACT FROM AUTHOR]